Does the melting temperature for mRNA to cDNA and Genomic DNA varies ? - pcr melting temperature calculator
What should be the annealing temperature for PCR and PCR-DNA - Genomic DNA
Wednesday, January 27, 2010
Pcr Melting Temperature Calculator Does The Melting Temperature For MRNA To CDNA And Genomic DNA Varies ?
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the temperature of hybridization or genomic DNA will be dependent on the GC and the content calculated as 4GC 2 AT. To determine the exact annealing temperature of the two, it would be better if you are running a gradient PCR to their first series with the temparature range above.
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